• Analyze Legionella in bulk and surface samples by the gold standard, culture.
  • Analysis includes total viable Legionella count and determination as Legionella pneumophila serogroup 1, Legionella pneumophila serogroup 2-14, or Legionella non-pneumophila species.
  • Pseudomonas aeruginosa counts are provided from decorative fountain and whirlpool/spa samples at no additional cost.
  • All Legionella reports include Ph.D. interpretations and recommendations based on sample location.
  • Individual sample sites are assigned a risk for Legionnaires Disease.

 

** Standard Processing: 7-10 days
** Rush Processing available: 4 days

 

LEGIONELLA REPORTS

sample type specific

All reports include Ph.D. Interpretations and Recommendations

Cooling Tower*

Bulk Liquid / Water

Limit of Detection = 10 CFU/ml

Swab

Limit of Detection = 20 CFU/swab

Ahu, Coils, Condensate

Bulk Liquid / Water

Limit of Detection = 10 CFU/ml

Swab

Limit of Detection = 20 CFU/swab

Closed Loop System

Bulk Liquid / Water

Limit of Detection = 10 CFU/ml

Swab

Limit of Detection = 20 CFU/swab

Potable Water

shower, faucet, hot water heater, drinking fountain, etc.

Bulk Liquid / Water

Limit of Detection = < 1 CFU/ml

Swab

Limit of Detection = 20 CFU/swab

Whirlpool, Spa, Hot Tub, Swimming Pool

with Pseudomonas aeruginosa screen

Bulk Liquid / Water

Limit of Detection = < 1 CFU/ml

Swab

Limit of Detection = 20 CFU/swab

Bulk Solid / Sand from Filter

Limit of Detection = calculated CFU/g

Decorative Fountain

with Pseudomonas aeruginosa screen

Bulk Liquid / Water

Limit of Detection = 10 CFU/ml

Swab

Limit of Detection = 20 CFU/swab

Eyewash

with Pseudomonas aeruginosa screen

Bulk Liquid / Water

Limit of Detection = < 1 CFU/ml

Metal Working/Cutting Fluid

Bulk Liquid / Water

Limit of Detection = < 1 CFU/ml

INDUSTRIAL FACILITY REPORT

All Facility reports include custom Ph.D. Interpretations and Recommendations

CRUISE SHIP REPORT

All cruise Ship reports include custom Ph.D.
Interpretations and Recommendations

 

Analytical Methods

Environmental samples are collected as either water samples or swabs taken from areas where water flows. Potable water samples are concentrated by filtration. To enhance Legionella recovery, samples are cultured on a selective media with antibiotics.

METHOD:

“Gold Standard” CDC approved testing procedure for
the isolation and confirmation of Legionella bacteria
in environmental samples (water or swab).

Sample Analysis:

Aerobic Heterotrophic Plate Count.

Viable Legionella detection and confirmation as being:

Legionellapneumophila serogroup 1

Legionellapneumophila serogroup 2-14

Legionella non-pneumophila species

CONFIRMATION:

Legionella isolates are confirmed by direct monoclonal antibody stain.

LAB REPORT:

Interpretations and recommendations provided.

LIMIT OF Detection:

10 CFU/ml for cooling tower samples

20 CFU/swab for swab samples

(CFU = Colony Forming Unit’s)

Additional:

Samples submitted for Legionella analysis may also be analyzed for other bacteria such as Pseudomonasaeruginosa or corrosive bacteria.

 

AIHA-LAP, LLC Accredited and CDC ELITE Proficient

Viable culture is considered to be the gold standard for assessing the presence of Legionella bacteria in environmental samples.

Environmental Safety Technologies, Inc. (EST) is certified by the Centers for Disease Control and Prevention (CDC) to be proficient in performing Legionella analysis by the ELITE (Environmental Legionella Isolation Techniques Evaluation) program.

EST was first awarded an ELITE Certificate of Proficiency during the pilot program in 2009 and has since maintained proficiency. While EST has been accredited by the AIHA-LAP, LLC Environmental Microbiology Laboratory Accreditation Program (AIHA-LAP, LLC EMLAP) since 2007 to perform microbiological testing for bacteria and fungi.

EST is also now one of the few laboratories that have been awarded accreditation by AIHA-LAP, LLC for the newly recognized field of testing specifically for environmental Legionella. These accreditations demonstrate that EST’s laboratory is competent to perform microbial and Legionella testing as proven by proficiency testing and through compliance to ISO 17025:2005 policies and requirements.